opa1  (Bioss)

Journal: Poultry Science

Article Title: Dihydromyricetin ameliorates lipopolysaccharide‒induced hepatic injury in chickens by activating the Nrf2/Keap1 pathway and regulating mitochondrial dynamics

doi: 10.1016/j.psj.2025.105034

Figure Lengend Snippet: Primer sequences used in this study

Article Snippet: The following primary antibodies were added to the membranes and incubated overnight at 4°C: anti‒Nrf2 (1:1000, No. WL02135, Wanleibio, China), anti‒Keap1 (1:1000, No. WL03285, Wanleibio, China), anti‒HO‒1 (1:500, No. WL02400, Wanleibio, China), anti‒NQO1 (1:500, No. WL04860, Wanleibio, China), and anti‒Drp1(1:500, No. WL03028, Wanleibio, China), anti‒Mnf1 (1:1000, No. bs-17693R, Bioss, China), anti‒Mnf2 (1:1000, No. bs-2988R, Bioss, China), anti‒Opa1 (1:1000, No. bsm-54144R, Bioss, China), anti‒Fis1 (1:1000, No. bsm-60551M, Bioss, China), and β-actin (1:1000, No. bs-0061R, Bioss, China).

Techniques: Sequencing

Journal: Poultry Science

Article Title: Dihydromyricetin ameliorates lipopolysaccharide‒induced hepatic injury in chickens by activating the Nrf2/Keap1 pathway and regulating mitochondrial dynamics

doi: 10.1016/j.psj.2025.105034

Figure Lengend Snippet: Changes in the levels of mitochondrial fusion division related factors in LPS‒induced chicken primary hepatocyte injury. (A) Mfn1, (B) Mfn2, (C) Opa1, (D) Fis1 and (E) Drp1 mRNA expression. (F) Protein bands of Opa1, Mfn1, Mfn2, Fis1 and Drp1. (G) Mfn1, (H) Mfn2, (I) Opa1, (J) Fis1 and (K) Drp1 protein levels. (L) The MMP of chicken hepatocytes. Magnification: 100 ×; bar: 100 μ m. The significance for comparisons between the control and model groups is noted as ** p < 0.01. The significance for comparisons between the model and DHM+LPS groups is noted as ## p < 0.01.

Article Snippet: The following primary antibodies were added to the membranes and incubated overnight at 4°C: anti‒Nrf2 (1:1000, No. WL02135, Wanleibio, China), anti‒Keap1 (1:1000, No. WL03285, Wanleibio, China), anti‒HO‒1 (1:500, No. WL02400, Wanleibio, China), anti‒NQO1 (1:500, No. WL04860, Wanleibio, China), and anti‒Drp1(1:500, No. WL03028, Wanleibio, China), anti‒Mnf1 (1:1000, No. bs-17693R, Bioss, China), anti‒Mnf2 (1:1000, No. bs-2988R, Bioss, China), anti‒Opa1 (1:1000, No. bsm-54144R, Bioss, China), anti‒Fis1 (1:1000, No. bsm-60551M, Bioss, China), and β-actin (1:1000, No. bs-0061R, Bioss, China).

Techniques: Expressing, Control

Journal: Poultry Science

Article Title: Dihydromyricetin ameliorates lipopolysaccharide‒induced hepatic injury in chickens by activating the Nrf2/Keap1 pathway and regulating mitochondrial dynamics

doi: 10.1016/j.psj.2025.105034

Figure Lengend Snippet: Changes in the expression of mitochondrial fusion division related factors in LPS‒induced hepatic injury. (A) Mfn1, (B) Mfn2, (C) Opa1, (D) Fis1 and (E) Drp1 mRNA expression. (F) Protein bands of Opa1, Mfn1, Mfn2, Fis1 and Drp1. (G) Mfn1, (H) Mfn2, (I) Opa1, (J) Fis1 and (K) Drp1 protein levels. (L) Morphology of hepatocyte mitochondria by ultramicropathology in chickens. Magnification: 20,000 ×; scale bar: 1 μm. The significance for comparisons between the control and model groups is noted as ** p < 0.01. The significance for comparisons between the model and DHM+LPS groups is noted as # p < 0.05 and ## p < 0.01.

Article Snippet: The following primary antibodies were added to the membranes and incubated overnight at 4°C: anti‒Nrf2 (1:1000, No. WL02135, Wanleibio, China), anti‒Keap1 (1:1000, No. WL03285, Wanleibio, China), anti‒HO‒1 (1:500, No. WL02400, Wanleibio, China), anti‒NQO1 (1:500, No. WL04860, Wanleibio, China), and anti‒Drp1(1:500, No. WL03028, Wanleibio, China), anti‒Mnf1 (1:1000, No. bs-17693R, Bioss, China), anti‒Mnf2 (1:1000, No. bs-2988R, Bioss, China), anti‒Opa1 (1:1000, No. bsm-54144R, Bioss, China), anti‒Fis1 (1:1000, No. bsm-60551M, Bioss, China), and β-actin (1:1000, No. bs-0061R, Bioss, China).

Techniques: Expressing, Control